A Rapid Means of Separating A14-125I-Insulin from Heterogeneously Labeled Insulin Molecules for Biologic Studies
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چکیده
منابع مشابه
A rapid means of separating A14-125I-insulin from heterogeneously labeled insulin molecules for biologic studies.
We have used two methods for the preparation of a highly homogeneous insulin with high specific activity. After iodination with chloramine T, the labeled peptides were retained on a disposable Sep Pak cartridge and subsequently eluted. The eluted labeled insulins were further purified by either DEAE cellulose or high performance liquid chromatography (HPLC) to separate A14-125I- from A19-125I-i...
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ژورنال
عنوان ژورنال: Diabetes
سال: 1982
ISSN: 0012-1797,1939-327X
DOI: 10.2337/diacare.31.12.1128